Syncytium formation is induced in the murine neuroblastoma cell cultures which produce pathogenic type G proteins of the rabies virus
Identifieur interne : 001B36 ( Main/Exploration ); précédent : 001B35; suivant : 001B37Syncytium formation is induced in the murine neuroblastoma cell cultures which produce pathogenic type G proteins of the rabies virus
Auteurs : Kinjiro Morimoto [Japon] ; Ya-Jin Ni [Japon] ; Akihiko Kawai [Japon]Source :
- Virology [ 0042-6822 ] ; 1992.
English descriptors
- Teeft :
- Amino, Amino acid, Amino acid substitution, Arginine, Butyrate, Cdna, Cell, Cell clones, Cell cultures, Cell density, Cell fusion, Cell lines, Cell surface, Cell types, Clone, Culture fluids, Culture medium, Dietzschold, Expression vector, Fusogenic, Gene expression, Giant cell formation, Glutamine, Glycoprotein, Host cells, Kawai, Monoclonal antibodies, Morimoto, Morphological changes, Multinucleated cells, Murine, Murine neuroblastoma, Mutagenesis technique, Mutant, Neuroblastoma, Neuroblastoma cells, Neuronal, Neuronal cells, Nonpathogenic, Nonpathogenic virus, Nonpathogenic viruses, Other hand, Pathogenic, Pathogenic virus, Protein, Protein synthesis, Putative fusogenic domain, Rabies, Rabies virus, Rabies virus glycoprotein, Relative amounts, Retroviral expression vector, Sodium, Sodium butyrate, Sodium butyrate treatment, Sodium cell cultures, Staining solution, Surface expression, Syncytium, Syncytium formation, Unpublished observations, Untreated, Viral, Viral invasion, Virology, Virulent, Virus, Wunner.
Abstract
Abstract: We investigated comparatively the interactions of host cells with two types of rabies virus G protein, an avirulent type G (Gln) and a virulent type G (Arg) protein, having glutamine and arginine at position 333, respectively. For this purpose, we established four types of cell lines (referred to as G(Gln)-NA, G(Arg)-NA, G(Gln)-BHK, and G(Arg)-BHK cells, respectively) by transfecting either the G(Gln)-cDNA or G(Arg)-cDNA into two kinds of cells, murine neuroblastoma C1300 (clone NA) and nonneuronal BHK-21. Both G(Gln)-NA and G(Arg)-NA cells produced G proteins when they were treated with 5 mM sodium butyrate, but only G(Arg)-NA cells formed syncytia at the neutral pH, which was suppressed by anti-G antiserum. The sodium butyrate-treated G(Arg)-NA cells fused also with sodium butyrate-treated NA cells under coculture conditions, but neither with untreated NA cells nor with BHK-21 cells. On the other hand, both G(Gln)-BHK and G(Arg)-BHK cells constitutively produced G proteins, but no syncytium was produced at the neutral pH. G(Arg)-BHK cells, however, formed syncytia with the sodium butyrate-treated NA cells when they were cocultured. These results suggest that only G(Arg) has a potential ability to produce syncytia of NA cells regardless of cell types by which G(Arg) protein was produced and also suggest that a certain cellular factor(s) is required for the syncytium formation, the factor(s) which is lacking in BHK-21 and untreated NA cells but is produced by the sodium butyrate-treated NA cells.
Url:
DOI: 10.1016/0042-6822(92)90696-M
Affiliations:
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xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Ni, Ya Jin" sort="Ni, Ya Jin" uniqKey="Ni Y" first="Ya-Jin" last="Ni">Ya-Jin Ni</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Kawai, Akihiko" sort="Kawai, Akihiko" uniqKey="Kawai A" first="Akihiko" 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type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid</term><term>Amino acid substitution</term><term>Arginine</term><term>Butyrate</term><term>Cdna</term><term>Cell</term><term>Cell clones</term><term>Cell cultures</term><term>Cell density</term><term>Cell fusion</term><term>Cell lines</term><term>Cell surface</term><term>Cell types</term><term>Clone</term><term>Culture fluids</term><term>Culture medium</term><term>Dietzschold</term><term>Expression vector</term><term>Fusogenic</term><term>Gene expression</term><term>Giant cell formation</term><term>Glutamine</term><term>Glycoprotein</term><term>Host cells</term><term>Kawai</term><term>Monoclonal antibodies</term><term>Morimoto</term><term>Morphological changes</term><term>Multinucleated cells</term><term>Murine</term><term>Murine neuroblastoma</term><term>Mutagenesis technique</term><term>Mutant</term><term>Neuroblastoma</term><term>Neuroblastoma cells</term><term>Neuronal</term><term>Neuronal cells</term><term>Nonpathogenic</term><term>Nonpathogenic virus</term><term>Nonpathogenic viruses</term><term>Other hand</term><term>Pathogenic</term><term>Pathogenic virus</term><term>Protein</term><term>Protein synthesis</term><term>Putative fusogenic domain</term><term>Rabies</term><term>Rabies virus</term><term>Rabies virus glycoprotein</term><term>Relative amounts</term><term>Retroviral expression vector</term><term>Sodium</term><term>Sodium butyrate</term><term>Sodium butyrate treatment</term><term>Sodium cell cultures</term><term>Staining solution</term><term>Surface expression</term><term>Syncytium</term><term>Syncytium formation</term><term>Unpublished observations</term><term>Untreated</term><term>Viral</term><term>Viral invasion</term><term>Virology</term><term>Virulent</term><term>Virus</term><term>Wunner</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We investigated comparatively the interactions of host cells with two types of rabies virus G protein, an avirulent type G (Gln) and a virulent type G (Arg) protein, having glutamine and arginine at position 333, respectively. For this purpose, we established four types of cell lines (referred to as G(Gln)-NA, G(Arg)-NA, G(Gln)-BHK, and G(Arg)-BHK cells, respectively) by transfecting either the G(Gln)-cDNA or G(Arg)-cDNA into two kinds of cells, murine neuroblastoma C1300 (clone NA) and nonneuronal BHK-21. Both G(Gln)-NA and G(Arg)-NA cells produced G proteins when they were treated with 5 mM sodium butyrate, but only G(Arg)-NA cells formed syncytia at the neutral pH, which was suppressed by anti-G antiserum. The sodium butyrate-treated G(Arg)-NA cells fused also with sodium butyrate-treated NA cells under coculture conditions, but neither with untreated NA cells nor with BHK-21 cells. On the other hand, both G(Gln)-BHK and G(Arg)-BHK cells constitutively produced G proteins, but no syncytium was produced at the neutral pH. G(Arg)-BHK cells, however, formed syncytia with the sodium butyrate-treated NA cells when they were cocultured. These results suggest that only G(Arg) has a potential ability to produce syncytia of NA cells regardless of cell types by which G(Arg) protein was produced and also suggest that a certain cellular factor(s) is required for the syncytium formation, the factor(s) which is lacking in BHK-21 and untreated NA cells but is produced by the sodium butyrate-treated NA cells.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><country name="Japon"><region name="Région du Kansai"><name sortKey="Morimoto, Kinjiro" sort="Morimoto, Kinjiro" uniqKey="Morimoto K" first="Kinjiro" last="Morimoto">Kinjiro Morimoto</name></region><name sortKey="Kawai, Akihiko" sort="Kawai, Akihiko" uniqKey="Kawai A" first="Akihiko" last="Kawai">Akihiko Kawai</name><name sortKey="Ni, Ya Jin" sort="Ni, Ya Jin" uniqKey="Ni Y" first="Ya-Jin" last="Ni">Ya-Jin Ni</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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